Specimen Requirements
Bone Marrow Core | 1-2cm in length | 10% Neutral Buffered Formalin (NBF) | Room Temperature | Indefinitely |
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Bone Marrow Clot | 10% Neutral Buffered Formalin (NBF) | Room Temperature | Indefinitely |
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Bone Marrow Aspirate Slides | Five (5) unstained, unfixed aspirate smears | Plastic Slide Holder | Room Temperature | Indefinitely |
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Histology (General H&E Stains) | Fresh Tissue | Primarily tumor tissue | 10% Neutral Buffered Formalin (NBF) | Room Temperature | Indefinitely |
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FFPE Tissue | FFPE Cassette | Room Temperature | Indefinitely | Include cold pack in extreme heat. | ||
Unstained Slides | Five (5) unstained, formalin-fixed slides | Plastic slide holder | Room Temperature | Indefinitely | Include cold pack in extreme heat. | |
Histology (IHC and Special Stains) | Fresh Tissue | Primarily tumor tissue | 10% Neutral Buffered Formalin (NBF) | Room Temperature | Indefinitely |
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FFPE Tissue | FFPE Cassette | Room Temperature | Indefinitely | Include cold pack in extreme heat. | ||
Unstained Slides | Two (2) unstained, formalin-fixed slides | Plastic slide holder | Room Temperature | Indefinitely | Include cold pack in extreme heat. | |
Histology - Breast Panel | FFPE Tissue | Block | FFPE Cassette | Room Temperature | Indefinitely |
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Unstained Slides | Ten (10) unstained, formalin-fixed slides | Plastic slide holder | Room Temperature | Indefinitely | Include cold pack in extreme heat. | |
Histology -Lymphoma, Myeloma and Neuroendocrine Panels | FFPE Tissue | Block | FFPE Cassette | Room Temperature | Indefinitely | Include cold pack in extreme heat. |
Unstained Slides | Ten (10) unstained, formalin-fixed slides | Plastic slide holder | Room Temperature | Indefinitely | Include cold pack in extreme heat. |
Flow Cytometry | Bone Marrow Aspirate | 1-2ml | Green top (sodium heparin) tube preferred, OR purple top (EDTA) tube | Room Temperature | 48 Hours | Hemolyzed, frozen or clotted samples may be rejected. |
Peripheral Blood | 2-5ml | Green top (sodium heparin) tube preferred, OR purple top (EDTA) tube | Room Temperature | 48 Hours | ||
Fresh Tissue | 1cm3 marble-sized piece of tissue | RPMI or sterile saline | Refrigerated | 24 hour |
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Fluid | 10ml | RPMI | Refrigerated | 24 Hours |
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Flow Cytometry - PNH | Peripheral Blood | 2-3ml | Green top (sodium heparin) tube preferred, OR purple top (EDTA) tube | Room Temperature | 48 Hours |
FISH
Bone Marrow & Peripheral Blood | 1~2ml | Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube, Smears or Cytospin | Room Temperature | 120 Hours | DO NOT FREEZE |
Formalin Fixed Paraffin Embedded Tissue (FFPET) | Block | FFPE Cassette | Room Temperature | Indefinitely | Include cold pack in extreme heat. |
Unstained, formalin-fixed slides. One (1) slide per FISH probe and one (1) additional for Pathological evaluation. | Plastic slide holder | Room Temperature | Indefinitely | Include cold pack in extreme heat. |
HER2 (ERBB2) by FISH
Formalin Fixed Paraffin Embedded Tissue (FFPET) | Block | FFPE Cassette | Room Temperature Include cold pack in extreme heat. | Indefinitely | Specimens subject to HER2 (ERBB2) testing should be fixed in 10% neutral buffered formalin for at least six hours and up to 72 hours. The volume of formalin should be at least 10 times the volume of the specimen. Decalcification solutions with strong acids should not be used. Specimens should be immersed in fixative within one hour of the biopsy or resection. Include cold ischemic time and fixation time on requisition. |
Unstained, formalin-fixed slides. Two (2) slides for FISH testing and one (1) additional for pathological evaluation. | Plastic slide holder | Room Temperature Include cold pack in extreme heat. | Indefinitely | Include cold pack in extreme heat. |
Bone Marrow (peripheral blood will be accepted in certain disease as CLL or if circulating blood containing more than 10% blasts) | Bone Marrow is ≥1.5 mL and the Peripheral blood is ≥2 mL if 10% or more blasts are present | Green top (Sodium Heparin) | Room Temperature | 72 Hours | No Frozen, Hemolyzed, Washed, in Syringes, in broken container or in different anti-coagulant other than sodium heparin. |
Voided Urine | Minimum of 33cc of urine | Urine Collection Kit | If Urine is not shipped immediately after collection, refrigerate immediately and ship via overnight courier within 24 hours. The preferred storage and shipping conditions are on ice packs, but specimens may be stored and shipped at temperatures up to 25°C | Must be processed within 48 hours of collection. | Follow Urine Collection Kit Instructions |